• Section 1 Clarifying problems, specifications and constraints

    Research has shown that the enzyme protease holds many benefits for industry and the medical community. Your team has been hired by the National Institute of Health (NIH) to design a model that will maximize protease production with the least amount of contamination. The budget is tight and it is important that the model produces the protein quickly while still retaining its enzymatic properties. If successful, this model could be used in underdeveloped countries where supplies are limited and the technologies to produce insulin in the Technology Park are non-existent.

     Your contract requires your team to:

    1. Choose a potential benefit of a protease.
    2. Determine the optimal model organism to produce the protein.
    3. Build a bioreactor to mass produce the protein with no contamination.
    4. Successfully grow the organism.
    5. Isolate the protein of interest.
    6. Test for the presence and activity of the protein using a variety of assays.
    7. Create a report for NIH Evaluation and determination of a future grant for further investigation in the amount of 1.2 million dollars.

    In addition to the task above, it is also important to the NIH that potential funding only go to team’s that display solid work ethics. Work ethics maintained in a work environment allows for a more productive, efficient, and reliable team. A member of the NIH team will randomly check in on team members to evaluate their work ethics using the Work Ethics Evaluation Rubric. This form will be kept in your personal file and will be used in your final evaluation for this project.

Course Safety and TrainingSection 2 Research and Investigate